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Monitoring and Controlling Biofilm Formation in Waters: Development of a Platform for Studying Bacterial-surface Interactions
Start Date 08/01/2006
End Date 07/31/2007
Primary Partner: Syracuse University
Primary Contact: Ren, Dacheng - Assistant Professor
Other Project Contacts:
Luk, Yan-Yeung - Co-Principal Investigator
Sangani, Ashok - Co-Principal Investigator
Project Type: CARTI I
Technical Description:
Assessment and control of bacterial contamination in waters is critically important for public health. Whereas the planktonic (free-swimming) cells are routinely monitored, the vast majority of bacteria exist attached to solid surfaces (known as biofilms) in natural environments and water distribution systems. Hence, they are not readily detected by routine measurement. In addition, biofilm cells are more resistant to environmental stresses and disinfectants than planktonic cells, and cause serious damages to water distribution and filtering systems by microbial-induced corrosion. Thus, the development of efficient and environmentally friendly anti-biofilm approaches is necessary. To achieve this long-term objective, this work is proposed as a preliminary study to develop a platform for studying bacteria-surface interactions and preventing biofilm formation. We propose the following specific aims:
Aim #l. Synthesize alkanethiolates presenting mannitol, mannose, and brominated furanone groups.
Aim #2. Modify the surfaces of gold and stainless steel with SAMs of alkanethiolates described above. Study biofilm formation of Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis on the engineered surfaces using confocal laser scanning microscope and imaging analysis.
Aim #3. Corroborate the results in Aim #2 by studying bacterial gene expression in contact with the surfaces.
Aim #4. Develop numerical simulations to aid in understanding the cells’ attachment and biofilm formation.
Expected Outcomes:
Develop a platform for studying bacteria-surface interactions and preventing biofilm formation.
Accomplishments:
The proposed project has been successfully finished through the collaboration of the PI and co-PI's labs and joint meetings were held frequently. The originally proposed goals were achieved and a significant amount of additional work (beyond the proposed aims) was accomplished. The research team is confident that the results will have significant impact on understanding and controlling microbial biofilm formation for improving water quality.
Benefits:
Applying technologies that analyze and detect bacterial contamination on surfaces in water sources will be an enhancement to the standard practices of detecting bacteria in water columns.
For more information:
http://www.lcs.syr.edu/faculty/ren/renlab/Research.htm
Publications:
Shuyu Hou, Erik A. Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren, "Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold." Applied and Environmental Engineering. 73:4300-4307 (2007).
Shuyu Hou, Erik A. Burton, Ricky Lei Wu, Yan-Yeung Luk and Dacheng Ren, "Prolonged Control of Patterned Biofilm Formation by Bio-inert Surface Chemistry". Chemical Communications. 2009, 1207-1209. Highlighted in Chemical Biology
Yongbin Han, Shuyu Hou, Karen Simon, Dacheng Ren, and Yan-Yeung Luk, “Identifying the Important Structural Elements in Brominated Furanones for Inhibiting Bacterial Biofilm Formation”, Bioorganic & Medicinal Chemistry Letters, 18: 1006-1010 (2008).
Erik A. Burton, Karen A. Simon, Shuyu Hou, Dacheng Ren, and Yan-Yeung Luk, “Molecular Gradients of Bio-inertness Reveal Mechanistic Difference between Mammalian Cell Adhesion and Bacterial Biofilm Formation”. Langmuir. 25: 1547-1553 (2009).
Miao Duo, Mi Zhang, Yan-Yeung Luk and Dacheng Ren, "Inhibition of Candida albicans Growth by Brominated Furanones". Applied Microbiology and Biotechnology. 85: 1551-1563 (2010).
Presentations:
Shuyu Hou, Erik Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren "Inhibiting E. Coli Biofilm Formation with Self Assembled Monolayers Presenting Functional Groups." AICHE Annual Meeting, San Francisco, CA, 2006.
Dacheng Ren, "Inhibiting Bacterial Multicellular Behavior: Challenges and Opportunities ", Syracuse University Physics Department, Syracuse, NY (invited seminar 2006).
Shuyu Hou, Eric A. Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren, "Controlling Escherichia coli Biofilm Formation with Self-Assembled Monolayers Presenting Functional Groups", SyracuseCoE Symposium (2006).
Miao Duo, Yongbin Han, Xianpeng Cai, Shuyu Hou, Yan-Yeung Luk, and Dacheng Ren "New Brominated Furanones as Biofilm Inhibitors." 107th ASM General Meeting, Toronto, Canada, 2007.
Shuyu Hou, Erik A. Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren "Controlling Bacterial Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold." 107th ASM General Meeting, Toronto, Canada, 2007.
Shuyu Hou, Erik A Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren (speaker) "Inhibiting bacterial biofilm formation by self-assembled monolayers of functional alkanethiols on gold", the 234 ACS Meeting, Boston, MA, 2007.
Miao Duo, YongBin Han, Shuyu Hou, Yan-Yeung Luk, and Dacheng Ren, "Synthesis and characterization of novel brominated furanones for controlling waterborne pathogens", SyracuseCoE Symposium (2007).
Shuyu Hou, Erik A. Burton, Karen A. Simon, Dustin Blodgett, Yan-Yeung Luk, and Dacheng Ren, "Controlling microbial adhesion and biofilm formation by self-assembled monolayers of functional alkanethiols", SyracuseCoE Symposium (2007).
Shuyu Hou and Dacheng Ren, “Patterned Biofilm Formation Reveals the Maximum Distance for Interaction Between Bacterial Clusters”, AICHE annual meeting, Nashville, TN (2009).
Patent:
Yan-Yeung Luk, Karen A. Simon, Dacheng Ren, "Biocatalytic Materials Built by Water-in-Water Emulsion", submitted (2007).
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