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Development of an Antigen-Responsive Hydro-Shell for Detecting Aqueous Toxins
Start Date 05/01/2007
End Date 04/30/2008
Primary Partner: Syracuse University
Primary Contact: Luk, Yan-Yeung - Assistant Professor
Other Project Contacts: Ren, Dacheng - Co-Principal Investigator
Project Type: CARTI II
Technical Description:
In this project, we will build a fundamentally new material of connected hydro-shell, of which the cross-links can be disassembled by the targeted antigen. This disruption of the cross-links will cause the shape of the hydro-shell to grossly change and be visually detected in real time without any instrumentation. The material is a development of polymerizing a water-in-water emulsion in which layers of water-solvated polymer encapsulate droplets of water-solvated lyotropic liquid crystals. When the layers of polymers are skillfully cross-linked and the liquid crystal is removed, highly porous hydrogels - thus hydro-shells - are obtained. By covalently incorporating antibodies and antigens in the polymers to function as cross-links for the formation of hydrogels, the free targeted antigen in solution can displace the existing cross-links and thus induce a readily visible collapse or disassembly of the hydro-shells. The novel detection mechanism will be applied to a model system of biotin and anti-biotin IgG, as well as to the whole cell of animal pathogen, Pseudomonas aeruginosa, and to the virulence factor, HrpW, secreted by the plant pathogen Pseudomonas syringae.
Expected Outcomes:
The development of a class of highly porous hydrogel materials that can detect in real time the presence of specific pathogenic bacteria and virulence factors (proteins).
Accomplishments:
The project has been successfully completed through the collaboration of the PI and co-PI's laboratories. Key results of each of the specific aims have been obtained with 2 peer-reviewed publications and 4 manuscripts under preparation. This study has also enabled the integration of laser diffraction and hydrogel with holographic grating as a powerful means to detect aqueous-borne toxins with higher sensitivity and without comprising the portability.
This project has established the key elements for building a new class of biosensors with fundamentally new sciences.
Benefits:
This process could provide near real-time detection of toxins in water sources.
For more information: http://techtransfer.syr.edu/Docs/100367 100372 Luk-Ren biotechnology-therapeutics MM.pdf
Publications:
Chiral Molecules with Polyhedral T, O or I Symmetry: Theoretical Solution to A Difficult Problem in Stereochemistry. Sri Kamesh Narasimhan, Xiaoying Lu and Yan-Yeung Luk Chirality 2008, 20(8) 878-884.
Identifying the important structural elements of brominated furanones for inhibiting biofilm formation by Escherichia coli. Yongbin Han, Shuyu Hou, Karen A. Simon, Dacheng Ren and Yan-Yeung Luk, Bioorg. Med. Chem. Lett., 2008, 18(3), 1006-1010.
Utilizing the high dielectric constant of water: efficient synthesis of amino acid-derivatized cyclobutenones Jun Li, Yongbin Han, Teresa B. Freedman, Shifa Zhu, Deborah J. Kerwood and Yan-Yeung Luk*, Tetrahedron Lett., 2008, 49, 2128-2131.
Patent applications:
Yan-Yeung Luk, Karen A. Simon, Dacheng Ren, "Biocatalytic Materials Built by Water-in-Water Emulsion", Filed (2008).
One-pot synthesis of protein-laden porous hydrogel
Image Credit: Yan-Yeung Luk, Michael Sponsler, Dacheng Ren; Syracuse University; November 2008.
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